How to Make Agar Slants

YouTube video

This video gives a walkthrough on how to make agar slants for preserving mushroom cultures. Agar slants are great for storing fungal cultures long-term. Culture slants can last months or years with no maintenance! Slants are much better than agar plates for long term storage of mushroom cultures because the air tight screw-cap prevents the culture from drying out over time. The easy method presented here uses a media dispensing bottle to rapidly fill many tubes without a mess! Filling each tube with a syringe or flask can be tedious and messy. Using the plastic dispensing bottle allows to you to seamlessly fill many tubes.

Media recipe:

  • 500ml water
  • 10g agar
  • 10-12g potato dextrose or malt extract

Click here for additional media recipes and more.

Related Videos

  • How to Grow Mushrooms in a Monotub
    How to Grow Mushrooms in a Monotub

    This video gives a quick 3-minute rundown on how to...

  • How to Make Mushroom Grain Spawn
    How to Make Mushroom Grain Spawn

    Grain spawn is fundamental to growing mushrooms. In this video,...

  • How to Pour Agar Plates (MEA)
    How to Pour Agar Plates (MEA)

    This video shows you everything you need to know to...

  • View All Videos

    I am a heading